Tyrosine phosphorylation of plant tubulin

0314548 - ÚMG 2009 RIV DE eng J - Journal Article
Blume, Y. B. - Yemets, A. - Sulimenko, Vadym - Sulimenko, Tetyana - Chan, J. - Lloyd, C. - Dráber, Pavel
Tyrosine phosphorylation of plant tubulin.
[Tyrozinová fosforylace tubulinu u rostlin.]
Planta. Roč. 229, č. 1 (2008), s. 143-150. ISSN 0032-0935. E-ISSN 1432-2048
R&D Projects: GA MŠMT LC545; GA ČR GA204/05/2375
Grant - others:EU(XE) INTAS 03-51-6459
Institutional research plan: CEZ:AV0Z50520514
Keywords : plant microtubules * tubulin * post-translation modifications
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.088, year: 2008

Phosphorylation of tubulins by protein tyrosine kinases plays an important role in animal cells. However, the role of tubulin tyrosine phosphorylation in plants is unknown and data on this tubulin modification are very limited. It was demonstrated that gamma-tubulins isolated by both immunoprecipitation and DEAE-chromatography is phosphorylated on tyrosine residues in cultured cells of Nicotiana tabacum. This opens up the possibility that tyrosine phosphorylation of tubulin could be involved in modulating the properties of plant microtubules.

Fosforylace tubulinů tyrozinovými kinázami hraje důležitou roli u živočišných buněk. Naše výsledky ukázaly, že v buněčné kultuře Nicotiana tabacum je gama-tubulin izolovaný pomocí imunoprecipitace nebo DEAE-chromatografie fosforylován na tyrozinu. Tato tubulinová fosforylace by mohla ovlivňovat vlastnosti mikrotubulů u rostlin.
Permanent Link: http://hdl.handle.net/11104/0165024