0312354 - MBÚ 2009 RIV NL eng J - Journal Article
Stodůlková, Eva - Novák, Petr - Deininger, S.-O. - Man, Petr - Čapková, Jana - Kavan, Daniel - Ivašková, E. - Flieger, Miroslav
LC MALDI-TOF MS/MS and LC ESI FTMS analyses of HLA-B27 associated peptides isolated from peripheral blood cells.
[LC MALDI-TOF MS/MS a LC ESI FTMS analýzy HLA-B27 asociovaných peptidů izolovaných z buněk periferní krve.]
Immunology Letters. Roč. 116, - (2008), s. 79-85. ISSN 0165-2478. E-ISSN 1879-0542
R&D Projects: GA MZd 1A8631
Institutional research plan: CEZ:AV0Z50200510; CEZ:AV0Z50520514
Keywords : hla-b27 * healthy blood donors
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.858, year: 2008 ; AIS: 0.814, rok: 2008

Peptides eluted from peripheral blood cells of HLA-B 2705 healthy donor were analyzed by LC MALDI MS/MS and LC ESI FTMS techniques. The sequences of 92 peptide ligands identified from one healthy blood donor by LC MALDI-TOF MS/MS were compared with those previously published. It was found that 18 sequences confirmed within 1 ppm mass error by LC ESI FTMS were already described and 3 of them matched with those previously reported from HLA-B 2705 splenocytes. Another 38 sequences validated within the same mass error were not found in SYFPEITHI database and are identified here for the first time. Finally, 36 sequences (5 sequences already published in SYFPEITHI database) were evaluated by LC MALDI-TOF MS/MS but no matches in the list of monoisotopic masses obtained from LC ESI FTMS were found

Peptidy eluované z krevních buněk HLA-B 2705 zdravých dárců byly analyzovány metodami LC MALDI MS/MS a LC ESI FTMS. Bylo identifikováno 92 peptidů z 1 zdravého dárce metodou LC MALDI-TOF MS/MS. 18 peptidových sekvencí získaných LC ESI FTMS (1 ppm) bylo známých a 3 z nich byly nalezeny u HLA-B 2705 splenocytů. Dalších 38 sekvencí nebylo nalezeno v SYFPEITHI databázi a jsou zde prezentovány poprvé. Nakonec, 36 sekvencí (5 peptidů publikovaných v SYFPEITHI databázi) získaných pomocí LC MALDI-TOF MS/MS nebyly detekovány metodou LC ESI FTMS
Permanent Link: http://hdl.handle.net/11104/0163436