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Analysis of the gene cluster coding for aldoxime and nitrile converting enzymes from Rhodococcus erythropolis A4
- 1.0307227 - MBÚ 2008 RIV SIGLE ES eng C - Conference Paper (international conference)
Volkova, O. - Knoppová, Monika - Kaplan, Ondřej - Kubáč, David - Nešvera, Jan - Martínková, Ludmila - Křen, Vladimír - Pátek, Miroslav
Analysis of the gene cluster coding for aldoxime and nitrile converting enzymes from Rhodococcus erythropolis A4.
[Analýza shluku genů kódujících enzymy konvertující aldoximy a nitrily u Rhodococcus erythropolis.]
International Symposium on Biocatalysis and Biotransformations /8./. Ovideo: Verlag, 2007, s. 163-163.
[International Symposium on Biocatalysis and Biotransformations /8./. Ovideo (ES), 08.07.2007-13.08.2007]
Institutional research plan: CEZ:AV0Z50200510
Keywords : rhodococcus erythropolis
Subject RIV: EE - Microbiology, Virology
The gene cluster including the genes oxd (aldoxime dehydratase), ami (amidase) and nha1-nha2 and subunits of nitrile hydratase) together with genes for transcriptional regulators from R. erythropolis A4 was isolated and characterized. The promoters of the cluster were cloned in the promoter-probe vector pEPR1 and their activity in R. erythropolis was analyzed using the fluorescence of the gfp reporter gene
Shluk genů zahrnující geny oxd (aldoximdehydratasa), ami (amidasa) and nha1-nha2 (podjednotka nitrilhydratasy) spolu s geny pro transkripční regulátory z R. erythropolis A4 byl izolován a charakterizován. Promotory genového shluku byly klonovány v promoter-probe vektoru pEPR1 a jejich aktivita byla analyzována s použitím měření fluorescence produkované reportérovým genem gfp
Permanent Link: http://hdl.handle.net/11104/0160049
Number of the records: 1