Fluorescence kinetic microscopy: 2D imaging of variable chlorophyll fluorescence from single algal cells with spectral resolution

0306892 - MBÚ 2008 RIV SIGLE CZ eng C - Conference Paper (international conference)
Prášil, Ondřej - Šetlíková, Eva - Veselka, D.
Fluorescence kinetic microscopy: 2D imaging of variable chlorophyll fluorescence from single algal cells with spectral resolution.
[Fluorescenční kinetická mikroskopie: 2D zobrazování variabilní fluorescence chlorofylu jednotlivých buněk řas se spektrálním rozlišením.]
Aquafluo - Chlorophyll Fluorescence in Aquatic Sciences Meeting. Nové Hrady: Springer, 2007, s. 75-75.
[Aquafluo - Chlorophyll Fluorescence in Aquatic Sciences Meeting. Nové Hrady (CZ), 28.05.2007-01.06.2007]
R&D Projects: GA MŠMT 1P05ME824
Institutional research plan: CEZ:AV0Z50200510
Keywords : fluorescence * kinetic * microscopy
Subject RIV: EE - Microbiology, Virology

The fluorescence kinetic microscopy (FKM) provides new dimension in application of chlorophyll fluorescence techniques. It combines classical fluorescence microscopy with 2D kinetic detection systems. FKM allows to examine photosynthesis of individual algal cells or on the cellular level of macroscopic structures. FKM is well suited to study heterogeneity in photosynthetic response among individual cells in algal cultures or in natural mixed populations. Special microscopic chambers allow to maintain live cells under optimal conditions for several hours or days and to follow evolution of photosynthesis in individual cells during the course of their growth cycle. Recent technical developments have allowed to determine FKM fluorescence kinetic with spectral resolution

Předmětem workshopu je seznámení se s technikou fluorescenční kinetické spektroskopie s 2D rozlišením, pro charakterizaci fotosyntetické aktivity jednotlivých buněk řas a sinic
Permanent Link: http://hdl.handle.net/11104/0159795