The fission yeast mitotic activator cdc25 and sucrose induce early flowering synergistically in the day-neutral Nicotiana tabacum cv. Samsun

0306001 - ÚEB 2008 RIV GB eng J - Journal Article
Teichmanová, M. - Mašková, P. - Vojvodová, P. - Krekule, Jan - Francis, D. - Lipavská, H.
The fission yeast mitotic activator cdc25 and sucrose induce early flowering synergistically in the day-neutral Nicotiana tabacum cv. Samsun.
[The fission yeast mitotic activator cdc25 and sucrose induce early flowering synergistically in the day-neutral Nicotiana tabacum cv. Samsun.]
New Phytologist. Roč. 176, č. 4 (2007), s. 804-812. ISSN 0028-646X. E-ISSN 1469-8137
Grant - others:Grantová agentura UK(CZ) 133/2000
Institutional research plan: CEZ:AV0Z50380511
Source of funding: V - Other public resources
Keywords : cdc25 gene * cell cycle regulation * day neutral tobacco
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 5.249, year: 2007

center dot Here, the tobacco (Nicotiana tabacum) day-neutral (DN) cv. Samsun transformed with the Schizosaccharomyces pombe mitotic activator gene Spcdc25 was used to study the onset of flowering. center dot Wild type (WT) and cdc25 plants were grown from seeds in vitro until they were 20 cm high. Apical and basal nodes were then subcultured repeatedly and the regenerated plants were used to document time to flowering and the number of leaves formed before flowering. Three sucrose treatments (3, 5 or 7% (weight/volume)) were used and measurements of leaf endogenous soluble carbohydrates were performed. center dot In the 3% treatment, cdc25 plants flowered but WT plants did not. The higher sucrose treatments enabled WT flowering; two-thirds of the plants flowered at 5%, while all plants flowered at 7% sucrose. However, in all treatments, cdc25 plants exhibited significantly earlier flowering and fewer leaves compared with wild type. Remarkably, a typical acropetal flowering gradient in WT plants did not occur in cdc25 plants. In cdc25 leaves, there were significantly higher amounts of endogenous sugars with a higher proportion of sucrose compared with WT. center dot Our data demonstrate that Spcdc25 expression and sucrose act synergistically to induce precocious flowering.

The fission yeast mitotic activator cdc25 and sucrose induce early flowering synergistically in the day-neutral Nicotiana tabacum cv. Samsun.
Permanent Link: http://hdl.handle.net/11104/0159116