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Maintenance of Meiotic Arrest in Bovine Oocytes In Vitro Using Butyrolactone I: Effects on Oocyte Ultrastructure and Nucleolus Function
- 1.0213515 - UZFG-Y 20023131 RIV US eng J - Journal Article
Motlík, Jan - Fair, T. - Hyttel, P. - Boland, M. - Lonergan, P.
Maintenance of Meiotic Arrest in Bovine Oocytes In Vitro Using Butyrolactone I: Effects on Oocyte Ultrastructure and Nucleolus Function.
Molecular Reproduction and Development. Roč. 62, - (2002), s. 375-386. ISSN 1040-452X. E-ISSN 1098-2795
R&D Projects: GA AV ČR KSK5052113
Grant - others:GA XE(XE) QLK3-CT-1999-00104
Keywords : meiosis * inhibition * cattle
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.322, year: 2002
In this study, we have shown that butyrolactone I (BL-I), a potent inhibitor of cyclindependent kinases, affects oocyte cytoplasmic morphology and nuclear function in terms of nucleolar ultrastructure and immunocytochemistry. Bovine oocytes were recovered from three classes of follicle size: 1,5-2, 2-3, and 3-6 mm. The oocytes were incubated for 40 hr with BL-I, and subsequently prcessed for transmission electron microscopy or immunocytochemistry.
Permanent Link: http://hdl.handle.net/11104/0109051
Number of the records: 1