Effect of bovine seminal ribonuclease and bovine pancreatic ribonuclease a on bovine oocyte maturation

0213250 - UZFG-Y 20003038 RIV US eng J - Journal Article
Slavík, Tomáš - Matoušek, Josef - Fulka, Josef - Raines, R. T.
Effect of bovine seminal ribonuclease and bovine pancreatic ribonuclease a on bovine oocyte maturation.
Journal of Experimental Zoology. Roč. 287, - (2000), s. 394-399. ISSN 0022-104X
R&D Projects: GA ČR GA523/96/0710; GA ČR GA524/99/0844; GA AV ČR KSK2052601
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.474, year: 2000

Bovine seminal ribonuclease (BS-RNase) contains the MxM (noncovalent dimer) and M=M (free monomer) in constant ratio. The aim of this work was to evaluate the effect of BS-RNase, its monomer and dimer forms, and also various mutants of this enzyme on meiotic completion in cattle oocytes. It was found that BS-RNase has irreversible effects on the meiotic maturation of bovine oocytes in vitro, particularly on the completion of meiosis. The effect of BS-RNase is dose-dependent. In medium supplemented with 1 mu g/ml, the results were comparable with those of the control (70% MII oocytes after 24 hr of culture). Whereas 5 mu g/ml reduced the number of MII oocytes to 50%, 10 and 25 mu g/ml arrested this process completely. The MxM form and RNase A at 5 mu g/ml inhibited the maturation rate by 71 and 48%, respectively, but a less significant effect was observed for the M=M form, or the carboxymethylated monomers MCM31 and MCM32 (21%, 16%, and 42% MII oocytes, respectively, in comparison with control). These data demonstrate that bovine ribonucleases can have variable detrimental effects on the maturation of bovine oocyte.
Permanent Link: http://hdl.handle.net/11104/0108788