Influence of water environment on biological specimens imaging in ESEM

0205651 - UPT-D 20030033 RIV HR eng C - Conference Paper (international conference)
Autrata, Rudolf - Neděla, Vilém - Horký, D.
Influence of water environment on biological specimens imaging in ESEM.
Proceedings of the 6th Multinational Congress on Microscopy - European Extension. Zagreb: Croatian Society for Electron Microscopy, 2003 - (Milat, O.; Ježek, D.), s. 497 - 498
[MCEM. Pula (HR), 01.06.2003-05.06.2003]
R&D Projects: GA ČR GA102/01/1271; GA AV ČR IBS2065107
Institutional research plan: CEZ:AV0Z2065902
Keywords : hydratation * wet specimen * pumping process
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering

Environmental scanning electron microscope (ESEM) allows the visualisation of specimens that are difficult or impossible to image in a conventional high vacuum SEM. Pressure in the specimen chamber ranges from 1 Pa over 2000 Pa, which permits observation of specimens of being free from charging artefacts and containing different volume of water. But there are some difficulties with the observation of highly wet biological specimens in their natural state because during a pre-pumping process of the specimen chamber an escape of water from the specimen occurs and thus its surface is damaged. The aim of this contribution is to find a method making possible to decrease water escape from the specimen during the initiation pumping process of the ESEM, immediatelly after the inserting of the specimen into the specimen chamber and during an image recording.
Permanent Link: http://hdl.handle.net/11104/0101264