Kinetics of the dimerization of retroviral proteases: The "fireman's grip" and dimerization

0194775 - UOCHB-X 20030081 RIV GB eng J - Journal Article
Ingr, Marek - Kondrová, Taťána - Stříšovský, Kvido - Majerová, E. - Konvalinka, Jan
Kinetics of the dimerization of retroviral proteases: The "fireman's grip" and dimerization.
Protein Science. Roč. 12, - (2003), s. 2173-2182. ISSN 0961-8368. E-ISSN 1469-896X
R&D Projects: GA MZd NI6339
Institutional research plan: CEZ:AV0Z4055905
Keywords : retroviral protease * dimerization * HIV protease
Subject RIV: CE - Biochemistry
Impact factor: 3.787, year: 2003

We prepared two pairs of the wild-type and mutant proteases from HIV and myeloblastosis-associated virus harboring either Ser or Thr in their Asp-Thr(Ser)-Gly triplet. The equilibrium dimerization constants differed by an order of magnitude within the relevant pairs. The dimer association contributes to this difference more than does the dissociation.
Permanent Link: http://hdl.handle.net/11104/0090446