Microsatellite region in the murine oncogene src - its primary structure and variability during evolution

0191701 - UMG-J 20033225 RIV CZ eng C - Conference Paper (international conference)
Fučík, Vladimír - Šindelka, Radek - Beran, Jaroslav - Ferjentsik, Zoltán - Jonák, Jiří
Microsatellite region in the murine oncogene src - its primary structure and variability during evolution.
Functional Genomics a Disease, Final programme and Abstract book. Praha: ESF IN FUNCTIONAL GENOMICS, 2003, s. 93.
[ESF IN FUNCTIONAL GENOMICS: CONFERENCE /1./. Praha (CZ), 14.05.2003-17.05.2003]
R&D Projects: GA ČR GA301/02/0408
Institutional research plan: CEZ:AV0Z5052915
Keywords : Mus musculus, c-src introns, microsatellite
Subject RIV: EB - Genetics ; Molecular Biology

In the fifth intron of the Mus musculus oncogene src a complex microsatellite was discovered that consists of a CA run and 31 bp apart followed by track of TG dinucleotides interrupted by extra Gs in specific intervals. As the 31 bp interlink can assume a hairpin structure, the whole region can be viewed as an imperfect inverted repeat. The microsatellite was sequenced in imbred lines of M. m. musculus and M. m. domesticus, as well as in mice of both subspecies trapped near the hybrid zone. Differences in the length of repeat regions, insertion/deletions and simple base changes characterize the strains. Hybrids between the two species could be identified by sequencing of cloned PCR products. The structure of the microsatellite in more distant species was found to be conspicuously different. Instead of frequent (CA)18-25, we can see only (CA)8 in M. spretus, CATGCGCCCCCCA in Mus pahari and CACCCCCC in Mus caroli. No similar structure is present in the corresponding human src intron.
Permanent Link: http://hdl.handle.net/11104/0087438