Isolation of non-heprin-binding and heparin-binding proteins of boar prostate

0191615 - UMG-J 20033121 RIV IE eng J - Journal Article
Maňásková, Pavla - Liberda, J. - Tichá, M. - Jonáková, Věra
Isolation of non-heprin-binding and heparin-binding proteins of boar prostate.
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 770, - (2002), s. 137-143. ISSN 1570-0232. E-ISSN 1873-376X.
[International Symposium /2./ - Separation in the BioSciences. Praha, 17.09.2001-20.09.2001]
R&D Projects: GA ČR GA303/99/0357; GA ČR GV524/96/K162
Institutional research plan: CEZ:AV0Z5052915
Keywords : isolation * prostatic proteins * heparin
Subject RIV: CE - Biochemistry
Impact factor: 1.913, year: 2002

Proteins of boar prostet secretion were separated by affinity chromatography on heparin-polyacrylamide to non-heparin-binding and heparin-binding protein fractions. These fractions were thwn subjected to RP HPLC. Elution profiles of fractions of prostate secretion were compared with non-heparin-binding and heparin-binding protein fractions of seminal plasma. Besides, the isolated proteins were characterized by SDS-PAGE. In protein fractions of prostate secretion, PSP I, PSP II, AQN 2 and AWN 1 spermadhesins were found in substantially lower amounts than in seminal plasma. On the countrary, beta-microseminoprotein was identified in abundant amounts in prostate secretion. Some seminal plasma proteins originating mainly inseminal vesicles could also be secreted by the prostatic gland. Beta-microseminoprotein was found to be produced mainly by the prostate.
Permanent Link: http://hdl.handle.net/11104/0087355