LIME: a new membrane raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling

0191603 - UMG-J 20033107 RIV US eng J - Journal Article
Brdičková, Naděžda - Brdička, Tomáš - Angelisová, Pavla - Horváth, Ondřej - Špička, Jiří - Hilgert, Ivan - Pačes, Jan - Simeoni, L. - Kliche, S. - Merten, C. - Schraven, B. - Hořejší, Václav
LIME: a new membrane raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.
Journal of Experimental Medicine. Roč. 198, č. 10 (2003), s. 1453-1462. ISSN 0022-1007. E-ISSN 1540-9538
R&D Projects: GA MŠMT LN00A079; GA MŠMT LN00A026
Grant - others:Wellcome Trust(GB) J1116W24Z
Institutional research plan: CEZ:AV0Z5052915
Keywords : immunology * T-lymphocyte * adaptor protein
Subject RIV: EC - Immunology
Impact factor: 15.302, year: 2003

Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src-family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. LIME thus appears to be involved in regulation of T cell activation by co-receptors.
Permanent Link: http://hdl.handle.net/11104/0087343