Calibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis

0191590 - UMG-J 20033091 RIV US eng J - Journal Article
Veselý, Pavel - Mikš, A. - Novák, J. - Boyde, A.
Calibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis.
Scanning. Roč. 25, - (2003), s. 230-239. ISSN 0161-0457. E-ISSN 1932-8745
R&D Projects: GA ČR GA304/99/0368
Institutional research plan: CEZ:AV0Z5052915
Keywords : fast intracellular motion * living cell ů video rate confocal laser scanning microscopy
Subject RIV: EA - Cell Biology
Impact factor: 0.733, year: 2003

Video Rate Confocal Laser Scanning Microscope (VRCLSM) Odyssey (Noran, Middleton, WI, USA) at the highest spatial and temporal resolution of back scattered light (BSL) imaging enabled regular observation of fast intracellular motion (FIM) first revealed in living neoplastic cells. However, the absence of an objective evaluation has hampered further study of the mechanisms and biological significance of FIM. A search for a suitable method led to correlation analysis. It was calibrated on Brownian motion and a known type of motion such as cell marginal ruffling compared with FIM. Therefore, several crucial incidences of FIM could be analyzed. Apart from an argument against viewing FIM as a manifestation of simple Brownian motion, the correlation analysis of FIM in the adjacent peripheries of a rat fibroblast and a K4 rat sarcoma cell confirmed the notion of higher and uneven distribution of velocity of FIM in a tumor cell so far shown in color coded images only.
Permanent Link: http://hdl.handle.net/11104/0087331