Expression of human eryhropoietin gene in the mammary gland of a transgenic mouse

0191355 - UMG-J 20010426 CZ eng J - Journal Article
Mikuš, T. - Malý, Petr - Poplštein, M. - Landa, Vladimír - Trefil, P. - Lidický, J.
Expression of human eryhropoietin gene in the mammary gland of a transgenic mouse.
Folia Biologica. Roč. 47, č. 6 (2001), s. 187-195. ISSN 0015-5500. E-ISSN 0015-5500
R&D Projects: GA MPO PP-Z1/09/96
Keywords : erythropoietin * recombinant protein * transgenic mouse
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.519, year: 2001

Whey acidic protein, WAP, is being recognized as the principal milk protein expressed in pregnant or lactating females of several mammalian species. We cloned the genomic fragment carrying the rabbit WAP gene locus from the phage rabbit genomic library and used the 8.5 Kb long 5 untraslated part of the rWAP gene to target expression of hEPO, cloned from the phage human genomic library, into the mammary gland of the mouse. The hybrid gene was injected into the mouse ova and transgenic mouse was produced.
Permanent Link: http://hdl.handle.net/11104/0087108