Exposure of lumenal microtubule sites after mild fixation

0191028 - UMG-J 20000130 RIV DE eng J - Journal Article
Dráberová, Eduarda - Viklický, Vladimír - Dráber, Pavel
Exposure of lumenal microtubule sites after mild fixation.
European Journal of Cell Biology. Roč. 79, č. 12 (2000), s. 982-985. ISSN 0171-9335. E-ISSN 1618-1298
R&D Projects: GA ČR GV312/96/K205; GA ČR GA204/98/1054
Institutional research plan: CEZ:AV0Z5052915
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.801, year: 2000

Docking the structure of tubulin dimer into a map of microtubules led to prediction that sites for tubulin acetylation are in the interior of microtubules. To assess the availability of acetylated alfa-tubulin for antibody, immunofluorescence on detergent-extracted cells, on cells fixed under various conditions and in microinjected cells was performed with antibodies of known epitope locations. The presented data indicate that acetylated alfa:Lys40 is not exposed on unfixed microtubules but that this region of lumenal microtubule surface becomes exposed under mild fixation conditions.
Permanent Link: http://hdl.handle.net/11104/0086788