0190909 - UMG-J 20000003 RIV CZ eng J - Journal Article
Čapková, Jana - Geussová, Gizela - Pěknicová, Jana
Monoclonal Antibody to Human Sperm Acrosomal Protein.
Folia Biologica. Roč. 46, č. 1 (2000), s. 55-57. ISSN 0015-5500. E-ISSN 0015-5500
R&D Projects: GA MZd IZ3911; GA ČR GV524/96/K162; GA MŠMT OE53/1
Institutional research plan: CEZ:AV0Z5052915
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.351, year: 2000

Monoclonal antibody designated Hs-14 was generated after immunization of BALB/c mice with acid extract of human sperm. In immunofluorescence the Hs-14 binds to acrosome of permeabilized sperm cells and recognizes some intra-acrosomal protein. Western blotting revealed that under non-reducing conditions the Hs-14 detects a protein with molecular mass of 220 kDa. Under reducing condition the Hs-14 recognizes peptide bands within the range from 55 kDa to 110 kDa. The antibody reacts also with sperm of other mammalian species. Using Hs-14 antibody it is possible to evaluate sperm pathology.
Permanent Link: http://hdl.handle.net/11104/0086672