Molecular sampling of hop stunt viroid (HSVd) from grapevines in hop production areas in the Czech Republic and hop protection

0189970 - UMBR-M 20033018 RIV CZ eng J - Journal Article
Matoušek, Jaroslav - Orctová, Lidmila - Patzak, J. - Svoboda, P. - Ludvíková, I.
Molecular sampling of hop stunt viroid (HSVd) from grapevines in hop production areas in the Czech Republic and hop protection.
Plant, Soil and Environment. Roč. 49, - (2003), s. 168-175. ISSN 1214-1178. E-ISSN 1805-9368
R&D Projects: GA MZe QC1183; GA AV ČR IBS5051014
Institutional research plan: CEZ:AV0Z5051902
Keywords : hop * viroid * hop protection
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.000, year: 2003

Molecular sampling of HSVd in grapevines in the environs of hop gardens was performed. Specific RT PCR primers were designed (to unambiguously distinguish between HLVd and HSVd infections) and used for detection and analysis of HSVd cDNAs from individual samples by thermodynamic methods, TGGE and cDNA heteroduplex analysis. We found that at least 70% of grapevine samples from locations close to hop gardens in Northern Bohemia were infected with HSVd forming populations containing quasispecies.
Permanent Link: http://hdl.handle.net/11104/0085767