Cloning and characterisation of chs-specific DNA and cDNA sequences from hop (Humulus lupulus L.)

0189900 - UMBR-M 20023006 RIV IE eng J - Journal Article
Matoušek, Jaroslav - Novák, Petr - Bříza, Jindřich - Patzak, J. - Niedermeierová, Hana
Cloning and characterisation of chs-specific DNA and cDNA sequences from hop (Humulus lupulus L.).
Plant Science. Roč. 62, - (2002), s. 1007-1018. ISSN 0168-9452. E-ISSN 1873-2259
R&D Projects: GA ČR GA521/99/1591
Keywords : hop * genetic manipulation
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.556, year: 2002

A complete sequence of chalcone synthase (CHS) gene from hop was cloned. The gene designated chs_H1 consists of two exons and one 187 bp intron. CHS protein predicted from chs_H1 cDNA has 42.5 kDa and retains conserved domains and residues including 26 amino acids at positions identical to those identified by crystallography as characteristic for catalytic domains of alfalfa CHS (EC 2.3.1.74). Cloned CHS_H1 protein shows specific CHS activity with 4-coumaroyl-CoA.
Permanent Link: http://hdl.handle.net/11104/0085697