DNA radiolysis in DNA-protein complex: a stochastic simulation of attack by hydroxyl radicals

0185871 - UJF-V 20035188 RIV GB eng J - Journal Article
Běgusová, Marie - Giliberto, S. - Gras, J. - Sy, D. - Charlier, M. - Spotheim Maurizot, M.
DNA radiolysis in DNA-protein complex: a stochastic simulation of attack by hydroxyl radicals.
International Journal of Radiation Biology. Roč. 79, č. 6 (2003), s. 385-391. ISSN 0955-3002. E-ISSN 1362-3095
R&D Projects: GA AV ČR IAA1048103
Institutional research plan: CEZ:AV0Z1048901
Keywords : radiolysis * DNA-protein complexes * hydroxyl radicals
Subject RIV: BO - Biophysics
Impact factor: 2.165, year: 2003

The paper presents an improved version of model Radack, a stochastic simulation procedure of radiolytic attack to DNA that takes into account the reactivity of each amino acid of a specifically bound protein with hydroxyl radicals. The model is applied to the natural lactose operator-repressor complex taking advantage of crystallography- and NMR-based structures recently reported in the PDB databank. The putative modeled structures of a natural lactose operator - repressor complex are discussed.
Permanent Link: http://hdl.handle.net/11104/0082224