0185191 - UJF-V 20000365 RIV GB eng J - Journal Article
Sjöström, A. - Tolmachev, V. - Koziorowski, J. - Lebeda, Ondřej - Einarsson, L. - Sjöberg, S. - Carlsson, J. - Lundquist, H.
Direct astatination of peptide, human epidermal growth factor, using nidocarborane as a prosthetic group.
Nuclear Medicine Communications. Roč. 21, - (2000), s. 594. ISSN 0143-3636. E-ISSN 1473-5628
Institutional research plan: CEZ:AV0Z1048901
Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders
Impact factor: 1.039, year: 2000

The aim of the study was to evaluate direct astatine labelling of proteins with the use of nido-carborane as prosthetic group for attachments of astatine. Methods:Human epidermal growth factor, hEGF, was conjugated to 7-(3-amino-propyl)-7,8-dicarbanido-undecaborate (-), ANC, using two different conjugation methods, glutaraldehyde crosslinking andcoupling via Traut`s reagent. The hEGF-ANC conjugates wereastatine labelled using the Chloramine-T method. The stability of the labelled compounds was tested by incubation at37.sup.o C.
Permanent Link: http://hdl.handle.net/11104/0081602