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The Application of Fluorescence Correlation Spectroscopy in Detecting DNA Condensation

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    0181391 - UFCH-W 20020055 RIV NL eng J - Journal Article
    Kral, T. - Langner, M. - Beneš, Martin - Baczynska, B. - Ugorski, M. - Hof, Martin
    The Application of Fluorescence Correlation Spectroscopy in Detecting DNA Condensation.
    Biophysical Chemistry. Roč. 95, č. 1 (2002), s. 135-144. ISSN 0301-4622. E-ISSN 1873-4200
    R&D Projects: GA MŠMT LN00A032
    Institutional research plan: CEZ:AV0Z4040901
    Keywords : plasmid condensation * spermine * fluorescence correlation spectroscopy
    Subject RIV: CF - Physical ; Theoretical Chemistry
    Impact factor: 1.494, year: 2002

    The application of fluorescence correlation spectroscopy (FCS) in characterizing conformational changes (condensation) of well-defined DNA Plasmids is shown. It became clear that the DNA molecule exhibits discrete conformational change between the coil and globule states with the addition of a small amount of cationic surfactant, spermine and hexadecyltrimethyl ammonium bromide (HTAB).

    Permanent Link: http://hdl.handle.net/11104/0077970

     
     

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