0173346 - UEM-P 20003069 RIV NL eng J - Journal Article
Benoist, P. - Feau, P. - Pliss, Artem - Voříšek, Josef - Antonelli, R. - Raška, Ivan - Duphil, M. D.
The yeast Ura2 protein that catalyses the first two steps of pyrimidines biosynthesis accumulates not in the nucleus but in the cytoplasm, as shown by immunocytochemistry and Ura2 - green fluorescent protein mapping.
Yeast. Roč. 16, - (2000), s. 1299-1312. ISSN 0749-503X. E-ISSN 1097-0061
R&D Projects: GA ČR GA302/99/0587
Grant - others:Wellcome Trust grant(XX) 049949/Z/97/Z/JMW/JPS/CG
Institutional research plan: CEZ:AV0Z5039906
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.825, year: 2000

The Ura2 protein was believed to be nucleoplasmic by means of three alternative approaches based on the detection of the protein itself: (a) indirect immunofluorescence of yeast protoplasts: (b) immunogold labelling of ultrathin sections of embedded yeast cells and (c) direct fluorescence of cells ewpressing an Ura2 - green fluorescent protein hybrid localize the bulk of Ura2 to the cytoplasm.
Permanent Link: http://hdl.handle.net/11104/0070380