Effect of 2-aminoindan-2-phosphonic acid on cell cycle progression in synchronous meristematic cells of Vicia faba roots

0172367 - UEB-Q 20033075 RIV FR eng J - Journal Article
Cvikrová, Milena - Binarová, Pavla - Cenklová, Věra - Eder, Josef - Doležel, Jaroslav - Macháčková, Ivana
Effect of 2-aminoindan-2-phosphonic acid on cell cycle progression in synchronous meristematic cells of Vicia faba roots.
Plant Science Bulletin. Roč. 164, č. 5 (2003), s. 823-832. ISSN 0032-0919. E-ISSN 1537-9752
R&D Projects: GA MŠMT LN00A081
Institutional research plan: CEZ:AV0Z5038910
Keywords : cell cycle * polyamines
Subject RIV: CE - Biochemistry

2-Aminoindan-2-phosphonic acid (AIP), a potent inhibitor of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), was applied to highly synchronous meristematic cells of Vicia faba roots containing cells in G1/S and G2/M cell cycle checkpoints. The inhibition resulted in a slight decline of the content of hydroxycinnamic derivatives 3.5 h after the AIP application. Flow cytometric analyses and BrdU immunolabelling of replicated cells showed that AIP applied in the G1-phase of the cell cycle delayed the progression through S-phase. The transient delay in DNA replication in treated cells was accompanied by accumulation of free spermidine and perchloric acid (PCA)-soluble spermidine conjugates. The cell cycle checkpoint G2/M appeared to be less sensitive to the inhibitor than G1/S. Mitotic activity of asynchronous root meristems was decreased only after the highest tested AIP concentration was applied (100 M) for 24 h. Significant increase in free putrescine and perchloric acid soluble-putrescine conjugates in cells after AIP-treatment might represent stress response in cells after AIP application. Increased PA conjugation suggests that it has an important role in the control of free PA contents in cells.
Permanent Link: http://hdl.handle.net/11104/0069406