0172289 - UEB-Q 20033002 RIV DE eng J - Journal Article
Roux, N. - Toloza, A. - Radecki, Z. - Zapata-Arias, F. J. - Doležel, Jaroslav
Rapid detection of aneuploidy in Musa using flow cytometry.
Plant Cell Reports. Roč. 21, - (2003), s. 483-490. ISSN 0721-7714. E-ISSN 1432-203X
R&D Projects: GA AV ČR IAA6038204
Institutional research plan: CEZ:AV0Z5038910
Keywords : banana * flow cytometry * nuclear DNA content
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.423, year: 2003

The procedure for rapid and convenient detection of aneuploidy in triploid Musa using DNA flow cytometry was reported. From a population of plants derived from gamma-irradiated shoot tips, plants were selected based on aberrant morphology and their chromosome numbers were counted. The nuclear DNA content of all plants were measured using flow cytometry. The classification based on flow cytometry fully agreed with the results obtained by chromosome counting. The results indicated that flow cytometry is a convenient and rapid method for the detection of aneuploidy in Musa.
Permanent Link: http://hdl.handle.net/11104/0069328