0172238 - UEB-Q 20023016 RIV GB eng J - Journal Article
Valárik, Miroslav - Šimková, Hana - Šafář, Jan - Doleželová, Marie - Doležel, Jaroslav
solation, characterization and chromosome localization of repetitive DNA sequences in bananas (Musa spp.).
Chromosome Research. Roč. 10, - (2002), s. 89-100. ISSN 0967-3849. E-ISSN 1573-6849
R&D Projects: GA AV ČR IAA6038204; GA AV ČR IBS5038104
Institutional research plan: CEZ:AV0Z5038910
Keywords : chromosome structure * genome size * in situ hybridization
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.828, year: 2002

Partial genomic DNA libraries were constructed in Musa acuminata and M. balbisiana and screened for clones carrying repeated sequences, and sequences carrying rDNA. Isolated clones were characterized in terms of copy number, genomic distribution in M. acuminata and M. balbisiana, and sequence similarity to known DNA sequences. Ribosomal RNA genes have been the most abundant sequences recovered. FISH with probes for DNA clones Radkal and Radkal, which carry different fragments of Musa 26S rDNA, and Radkal, for which no homology with known DNA sequences has been found, resulted in clear signals at secondary constrictions. Only one clone carrying 5S rDNA, named Radkal, has been recovered. All remaining DNA clones exhibited more or less pronounced clustering at centromeric regions. The study revealed small differences in genomic distribution of repetitive DNA sequences between M. acuminata and M. balbisiana, the only exception being the 5S rDNA where the two Musa clones under study differed in the number of sites.
Permanent Link: http://hdl.handle.net/11104/0069277