Isolation of proteins comprising native gene-specific messenger ribonucleoprotein particles using paramagnetic beads

0172200 - UEB-Q 20013079 RIV IE eng J - Journal Article
Honys, David
Isolation of proteins comprising native gene-specific messenger ribonucleoprotein particles using paramagnetic beads.
Plant Science. Roč. 161, č. 3 (2001), s. 605-611. ISSN 0168-9452. E-ISSN 1873-2259
R&D Projects: GA ČR GP204/01/D073
Institutional research plan: CEZ:AV0Z5038910
Keywords : Magnetic isolation * Protein purification * RNA-binding proteins
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.384, year: 2001

A method for the rapid isolation of proteins comprising native messenger ribonucleoprotein particles (mRNPs), that utilises streptavidin-coated paramagnetic beads, coupled with biotinylated oligodeoxyribonucleotides, to pull down general and transcript-specific mRNP complexes from crude cell-free extracts, is described. Two biotinylated oligo probes were tested for their efficacy in isolating mRNPs from tobacco pollen extracts; a 25-mer oligo(dT) probe for the isolation of total mRNPs, and a transcript-specific oligo complementary to the pollen-specific ntp303 mRNA sequence.
Permanent Link: http://hdl.handle.net/11104/0069242