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Magnetic alginate microparticles for purification of .alpha.-amylases
- 1.0170718 - UEK-B 20033023 RIV NL eng J - Journal Article
Šafaříková, Miroslava - Roy, I. - Gupta, M. N. - Šafařík, Ivo
Magnetic alginate microparticles for purification of .alpha.-amylases.
Journal of Biotechnology. Roč. 105, - (2003), s. 255-260. ISSN 0168-1656. E-ISSN 1873-4863
R&D Projects: GA MŠMT OC 523.80; GA AV ČR IBS6087204
Institutional research plan: CEZ:AV0Z6087904
Keywords : alginate * ferrofluid * amalyses
Subject RIV: CE - Biochemistry
Impact factor: 2.543, year: 2003
Spherical magnetic alginate microparticles (25 - 60 .micro.m in diameter) were prepared using the microemulsion system, with water saturated 1-pentanol as the organic phase. The limited solubility of 1-pentanol in water enabled simple removal of the organic solvent from the prepared beads with water solutions. The prepared alginate microparticles were used as magnetic affinity adsorbents for specific purification of .alpha.-amylases. Enzyme activity was eluted by 1.0 M maltose. Alpha-amylases from Bacillus amyloliquefaciens and porcine pancreatic acetone powder were purified 9- and 12-fold with 88 and 96 % activity recovery, respectively.
Permanent Link: http://hdl.handle.net/11104/0067800
Number of the records: 1