A biosensor for the detection of triazine and phenylurea herbicides designed using Photosystem II coupled to a screen-printed electrode

0170636 - UEK-B 20013112 RIV US eng J - Journal Article
Koblížek, Michal - Malý, Jan - Masojídek, Jiří - Komenda, Josef - Kučera, T. - Giardi, M. G. - Mattoo, A. K. - Pilloton, R.
A biosensor for the detection of triazine and phenylurea herbicides designed using Photosystem II coupled to a screen-printed electrode.
Biotechnology and Bioengineering. Roč. 78, č. 1 (2002), s. 110-116. ISSN 0006-3592. E-ISSN 1097-0290
Institutional research plan: CEZ:AV0Z6087904
Keywords : atrazine,diuron,flow-cell system
Subject RIV: EH - Ecology, Behaviour
Impact factor: 2.211, year: 2002

A biosensor for the detection of triazine- and phenylurea-type herbicides was constructed using isolated Photosystem II (PS II) complexes as a biosensing element. PSII isolated from the thermophilic cyanobacterium Synechococcus elongatus was immobilized on the surface of a screen-printed sensor composed of a graphite working electrode and Ag/AgCl reference electrode deposited on a polymeric substrate. The biosensor was mounted in a flow microcell with illumination. The principle of the detection was based on the fact that herbicides selectively block PSII electron transport activity in a concentration-dependent manner. Changes of the activity were registered amperometrically as the rate of photoreduction of an artificial electron acceptor. The setup resulted in a reusable herbicide biosensor with a good stability (half-life of 24 h) and limit of detection of approximately 10-9 M for diuron, atrazine and simazine.
Permanent Link: http://hdl.handle.net/11104/0067720