Effect of the salivary gland and midgut extracts from Ixodes ricinus and Dermacentor reticulatus (Acari : Ixodidae) on the growth of Borrelia garinii in vitro

0164467 - UBO-W 20030034 RIV CZ eng J - Journal Article
Rudolf, Ivo - Hubálek, Zdeněk
Effect of the salivary gland and midgut extracts from Ixodes ricinus and Dermacentor reticulatus (Acari : Ixodidae) on the growth of Borrelia garinii in vitro.
Folia Parasitologica. Roč. 50, č. 2 (2003), s. 159-160. ISSN 0015-5683. E-ISSN 1803-6465
R&D Projects: GA AV ČR IAA6022001; GA ČR GA206/00/1204; GA ČR GA206/03/0726
Institutional research plan: CEZ:AV0Z6093917
Keywords : Ixodes ricinus * Dermacentor reticulatus
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.469, year: 2003
http://folia.paru.cas.cz/pdfs/showpdf.php?pdf=20661

In our study we examined the effect of salivary gland extract (SGE) and midgut extract (MGE) from Ixodes ricinus and Dermacentor reticulatus on the growth, motility and morphology of the B. garinii spirochete in vitro. Concentration of motile spirochetes (the number of motile cells/ml of medium) was determined at specific intervals using darkfield microscopy. In I. ricinus, the concentration of motile spirochetes increased significantly between the days 2 and 11 post inoculation (p.i.) with both SGE and MGE compared to control (C). In D. reticulatus, a significant increase in concentration of motile spirochetes was only detected with SGE (compared to control) on the day 5 p.i., while a marked decrease in concentration of motile spirochetes was observed on day 9 p.i. due to MGE effect, and on day 12 p.i. due to the effect of both extracts compared to control. The effect of SGE and MGE on the growth of B. garinii in vitro differed between the two ticks species tested: while the extracts derived from I. ricinus (a competent vector for Lyme borreliosis) stimulated significantly the growth, those from D. reticulatus (a non-competent species) did not affect the growth of borreliae markedly, or even inhibited their growth on days 9 (MGE) and 12 p.i. (MGE and SGE). Our results therefore indicate that the tick compartment extracts surprisingly need not be inhibitory for pathogen survival in the body of even non-competent tick species like D. reticulatus in a short-term exposure.
Permanent Link: http://hdl.handle.net/11104/0061686