Chloride present in biological samples as a tool for enhancement of sensitivity in capillary zone electrophoretic analysis of anionic trace analytes

0162494 - UIACH-O 20033055 RIV DE eng J - Journal Article
Křivánková, Ludmila - Pantůčková, Pavla - Gebauer, Petr - Boček, Petr - Caslavska, J. - Thormann, W.
Chloride present in biological samples as a tool for enhancement of sensitivity in capillary zone electrophoretic analysis of anionic trace analytes.
Electrophoresis. Roč. 24, č. 3 (2003), s. 505-517. ISSN 0173-0835. E-ISSN 1522-2683
R&D Projects: GA ČR GA203/02/0023; GA ČR GA203/01/0401; GA AV ČR IAA4031103
Institutional research plan: CEZ:AV0Z4031919
Keywords : acetoacetate * capillary zone electrophoresis * chloride stacking effects
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.040, year: 2003

For stacking and separation of anionic trace analytes in biological samples, the presence of chloride is shown to be important to balance out effects of other macrocomponents that act against isotachophoretic stacking. Having acetoacetate, malate, citrate and some drug metabolites in untreated human serum samples, the stacking mechanism of these compounds in an electrolyte system comprising 5 mM mandelic acid + e-aminocaproic acid, pH 3.8, is explained. Insight into the separation process is given both with computer simulations and experiments. For selected analytes, the effect of chloride concentration on quantitative evaluation, sensitivity and limit of detection is demonstrated as well.
Permanent Link: http://hdl.handle.net/11104/0059807