Stacking phenomena in electromigration: From basic principles to practical procedures

0162485 - UIACH-O 20033048 RIV DE eng J - Journal Article
Urbánek, Marek - Křivánková, Ludmila - Boček, Petr
Stacking phenomena in electromigration: From basic principles to practical procedures.
Electrophoresis. Roč. 24, č. 3 (2003), s. 466-485. ISSN 0173-0835. E-ISSN 1522-2683
R&D Projects: GA ČR GA203/02/0023; GA ČR GA203/01/0401; GA AV ČR IAA4031103
Institutional research plan: CEZ:AV0Z4031919
Keywords : stacking * capillary zone electrophoresis * isotachophoresis
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.040, year: 2003

The review describes principles of procedures and techniques used in capillary zone electrophoresis for enhancement of sensitivity that are based on increasing analyte mass in its zone during the electromigration process, for which the term stacking is generally used. Attention is paid to intrinsic stacking in samples with low conductivity, transient isotachophoretic stacking applied in samples with high conductivity, and sweeping in micellar electrokinetic chromatography. Principles of these stacking schemes are explained, new procedures and instrumental arrangements are discussed, and all contributions involving stacking principles that have been published since the year 2000 are surveyed.
Permanent Link: http://hdl.handle.net/11104/0059798