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A miniaturized multichamber solution isoelectric focusing device for separation of protein digests
- 1.0162432 - UIACH-O 20020084 RIV DE eng J - Journal Article
Tan, A. - Pashkova, A. - Zang, L. - Foret, František - Karger, B. L.
A miniaturized multichamber solution isoelectric focusing device for separation of protein digests.
Electrophoresis. Roč. 23, č. 20 (2002), s. 3599-3607. ISSN 0173-0835. E-ISSN 1522-2683
R&D Projects: GA AV ČR IBS4031209
Institutional research plan: CEZ:AV0Z4031919
Keywords : solution IEF * peptides * mass spectrometry
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.325, year: 2002
A miniaturized multichamber device was constructed for solution isoelectric focusing (IEF) separation of complex peptide mixtures. A variety of standard peptide mixtures and tryptic digests of proteins were separated. The IEF separation provided concentrated and desalted fractions, suitable for an additional separation liquid chromatography, capillary electrophoresis (LC, CE) or mass spectrometry (MS) detection without additional sample cleanup.
Permanent Link: http://hdl.handle.net/11104/0059747
Number of the records: 1