Proteomics of barley proteins by combination of in-gel digestion and MALDI-TOF MS

0162313 - UIACH-O 20010064 RIV CZ eng C - Conference Paper (international conference)
Řehulka, Pavel - Chmelík, Josef - Mayrhofer, C. - Allmaier, G.
Proteomics of barley proteins by combination of in-gel digestion and MALDI-TOF MS.
2nd International Symposium Separations in the BioSciences SBS 2001. Praha: Czech Chemical Society, Chromatography and Electrophoretic Group, 2001, s. 133. ISBN 80-7080-437-8.
[International Symposium Separations in the BioSciences SBS 2001 /2./. Praha (CZ), 17.09.2001-20.09.2001]
R&D Projects: GA ČR GA521/99/1576
Grant - others:Austrian-Czech Republic Collaboration Grant(AT) II/4
Institutional research plan: CEZ:AV0Z4031919
Keywords : proteomics * protein identification * barley
Subject RIV: CB - Analytical Chemistry, Separation

It is shown that combination of gel electrophoresis, matrix assisted laser desorption/ionization time-of-flight mass spectrometry and database searching is a suitable procedure for rapid protein identification. This approach let us to identify some proteins from barley leaves and grains. Identification of barley proteins can be useful for characterization of varieties of malting barley which is important especially in food and brewing industry.
Permanent Link: http://hdl.handle.net/11104/0059631