Electromigration behavior of SSDNA fragments in highly alkaline solutions of linear polyacrylamide

0162294 - UIACH-O 20010045 RIV US eng C - Conference Paper (international conference)
Malá, Zdeňka - Klepárník, Karel - Boček, Petr
Electromigration behavior of SSDNA fragments in highly alkaline solutions of linear polyacrylamide.
HPCE 2001. 14th International Symposium on Microscale Separations and Analysis Includes mini-symposia on Genomics and Proteomics. Boston: CaSSS, 2001, s. 156.
[HPCE 2001. International Symposium on Microscale Separations and Analysis Includes mini-symposia on Genomics and Proteomics /14./. Boston (US), 13.01.2001-18.01.2001]
R&D Projects: GA ČR GA203/00/0772; GA ČR GA303/00/0928
Institutional research plan: CEZ:AV0Z4031919
Keywords : DNA sequencing * bare capillary * alkaline solution
Subject RIV: CB - Analytical Chemistry, Separation

Bare fused silica capillaries filled with highly alkaline solutions of linear polyacrylamide provide fast and accurate DNA analyses. These electrolyte systems have successfully been used for the diagnostic analyses. To demonstrate the positive properties of the medium, separations performed under various operational conditions are presented. The experimental results are compared to theoretical predictions.
Permanent Link: http://hdl.handle.net/11104/0059613