DNA denaturing capillary electrophoresis in highly alkaline solutions

0162214 - UIACH-O 200028 RIV DE eng C - Conference Paper (international conference)
Klepárník, Karel - Malá, Zdeňka - Boček, Petr
DNA denaturing capillary electrophoresis in highly alkaline solutions.
13th International Symposium on High Performance Capillary Electrophoresis and Related Microscale Techniques. Final Program. Book of Abstracts. Saarbrücken, 2000, s. 37.
[International Symposium on High Performance Capillary Electrophoresis and Related Microscale Techniques /13./. Saarbrücken (DE), 20.02.2000-24.02.2000]
Institutional research plan: CEZ:AV0Z4031919
Subject RIV: CB - Analytical Chemistry, Separation

Denaturing capillary electrophoresis provides much faster and more selective separations of DNA fragments than electrophoresis under native conditions. We have developed a highly alkaline electrolyte system with remarkable properties such as extraordinary denaturing power, high separation and low electroosmotic flow mobility in bare fused-silica capillaries. Thus, the analyses of a microsatellite repeat polymorphism in Endothelin 1 gene under denaturing conditions in short capillaries (2.5 cm) with laser-induced fluorescence detection were accomplished in 42 seconds.
Permanent Link: http://hdl.handle.net/11104/0059533