0142136 - FGU-C 20010190 RIV NL eng J - Journal Article
Doležal, Vladimír - Lisá, Věra - Diebler, M. F. - Kašparová, Jana - Tuček, Stanislav
Differentiation of NG108-15 cells induced by the combined presence of dbcAMP and dexamethasone brings about the expression of N and P/Q types of calcium channels and the inhibitory influence of muscarinic receptors on calcium influx.
Brain Research. Roč. 910, 1-2 (2001), s. 134-141. ISSN 0006-8993. E-ISSN 1872-6240
R&D Projects: GA ČR GA305/98/0469; GA ČR GA305/01/0283; GA MZd NF5183
Institutional research plan: CEZ:AV0Z5011922
Keywords : NG108-15 cell line * cAMP * calcium channels
Subject RIV: ED - Physiology
Impact factor: 2.489, year: 2001

Differentiation of cholinergic cell line NG108-15 induced by a combination of dibutyryl cyclic AMP (dbcAMP) and dexamethasone enhances the cholinergic phenotype of the cells more than that induced by either agent alone. We investigated the effect of treatment with dbcAMP and dexamethasone on potassium depolarization-evoked influx of calcium and its regulation by the muscarinic agonist carbachol. Depolarization of control cells and of cells differentiated in the presence of dbcAMP or dexamethasone alone, or in the combined presence of dbcAMP and dexamethasone induced, respectively, 2.2, 4.3, 2.7 and 10.7 fold increases of the resting [Ca2+]i. Dexamethasone alone and the combination of dbcAMP and dexamethasone augmented the number of muscarinic receptors by 25 and 40
Permanent Link: http://hdl.handle.net/11104/0039845