Red pigment-concentrating hormone is not limited to crustaceans

0136034 - ENTU-I 20033058 RIV US eng J - Journal Article
Gäde, G. - Auerswald, L. - Šimek, Petr - Marcoa, H. G. - Kodrík, Dalibor
Red pigment-concentrating hormone is not limited to crustaceans.
Biochemical and Biophysical Research Communications. Roč. 309, - (2003), s. 967-973. ISSN 0006-291X. E-ISSN 1090-2104
R&D Projects: GA AV ČR IPP1050128; GA AV ČR IAA6007202
Institutional research plan: CEZ:AV0Z5007907
Keywords : Arthropods * Crustaceans * insects
Subject RIV: ED - Physiology
Impact factor: 2.836, year: 2003

A peptide that was previously assumed to occur exclusively in crustaceans is found in the corpora cardiaca of the stinkbug, Nezara viridula. The sequence of the peptide was deduced from the multiple MSN electrospray mass data as that of an octapeptide: pGluůIle/LeuůAsnůPheůSerůProůGlyůTrp amide. This peptide with Leu at position 2 is known as crustacean red pigment-concentrating hormone and code-named Panbo-RPCH. The ambiguity about the amino acid at position 2, Leu or Ile, was solved by isolating the peptide in a single-step by reversed-phase HPLC and establishing co-elution with authentic Panbo-RPCH but not with the Ile2-analog. When injected into stinkbugs, synthetic Panbo-RPCH elicited an increase of lipids in the haemolymph. Thus, it is assumed that Panbo-RPCH functions in the stinkbug as a lipid-mobilizing hormone.
Permanent Link: http://hdl.handle.net/11104/0033795