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A quantitative study of adipokinetic hormone of the firebug Pyrrhocoris apterus
- 1.0135864 - ENTU-I 20023083 RIV GB eng J - Journal Article
Goldsworthy, G. J. - Kodrík, Dalibor - Comley, R. - Lightfoot, M.
A quantitative study of adipokinetic hormone of the firebug Pyrrhocoris apterus.
Journal of Insect Physiology. Roč. 48, - (2002), s. 1103-1109. ISSN 0022-1910. E-ISSN 1879-1611
R&D Projects: GA AV ČR IAA6007202
Institutional research plan: CEZ:AV0Z5007907
Keywords : Adipokinetic hormone * ELISA * Pyrrhocoris apterus
Subject RIV: ED - Physiology
Impact factor: 1.789, year: 2002
The development of an enzyme-linked immunoassay (ELISA) for the adipokinetic neuropeptide hormone, Pya-AKH, from the firebug Pyrrhocoris apterus L. is described. The ELISA measures as little as 20 fmol of Pya-AKH. Tested against a range of synthetic peptides, the assay has a high sensitivity for peptides containing the C-terminal motif FTPNWamide. The amounts of Pya-AKH in the brain, corpora cardiaca, suboesophageal ganglia, and fused thoracic and abdominal ganglionic mass are very small, with only the corpora cardiaca containing appreciable levels of the hormone (ca. 4pmol per bug).
Permanent Link: http://hdl.handle.net/11104/0033640
Number of the records: 1