Genetic and functional analysis of DD44, a sex-linked gene from the dioecious plant Silene latifolia, provides clues to early events in sex chromosome evolution

0127063 - BFU-R 20033151 RIV US eng J - Journal Article
Moore, R. C. - Kozyreva, O. - Lebel-Hardenack, S. - Široký, Jiří - Hobza, Roman - Vyskot, Boris - Grant, S. R.
Genetic and functional analysis of DD44, a sex-linked gene from the dioecious plant Silene latifolia, provides clues to early events in sex chromosome evolution.
Genetics. Roč. 163, č. 1 (2003), s. 321-334. ISSN 0016-6731. E-ISSN 1943-2631
R&D Projects: GA ČR GA204/02/0417
Grant - others:National Science Foundation(US) MCB-9816864; National Institutes of Health(US) F32 GM20891-01
Institutional research plan: CEZ:AV0Z5004920
Keywords : dioecious plant * Silene latifolia * sex chromosome
Subject RIV: BO - Biophysics
Impact factor: 4.276, year: 2003

Here, we present the genetic and physical mapping, expression analysis, and molecular evolutionary analysis of a sex-linked gene from S. latifolia, DD44 (Differential Display 44). DD44 is homologous to the oligomycin sensitivity-conferring protein, an essential component of the mitochondrial ATP synthase, and is ubiquitously expressed in both sexes. We have been able to genetically map DD44 to a region of the Y chromosome that is genetically linked to the carpel-suppressing locus. Although we have physically mapped DD44 to the distal end of the long arm of the X chromosome using fluorescence in situ hybridization, DD44 maps to the opposite arm of the Y chromosome as determined by our genetic map. These data suggest that chromosomal rearrangements have occurred on the Y chromosome, which may have contributed to the genetic isolation of the Y chromosome. We discuss the implications of these results with respect to the structural and functional evolution of the S. latifolia Y chromosome.
Permanent Link: http://hdl.handle.net/11104/0025286