Nuclear and territorial topography of chromosome telomeres in human lymphocytes

0127047 - BFU-R 20033135 RIV US eng J - Journal Article
Amrichová, J. - Lukášová, Emilie - Kozubek, Stanislav - Kozubek, Michal
Nuclear and territorial topography of chromosome telomeres in human lymphocytes.
Experimental Cell Research. Roč. 289, č. 1 (2003), s. 11-26. ISSN 0014-4827. E-ISSN 1090-2422
R&D Projects: GA MZd NC5955; GA MZd NC6987; GA AV ČR IBS5004010; GA ČR GA202/02/0804; GA ČR GA301/01/0186
Institutional research plan: CEZ:AV0Z5004920
Keywords : nuclear architecture * telomere * centromere
Subject RIV: BO - Biophysics
Impact factor: 3.949, year: 2003

Nuclear and territorial positioning of p- and q-telomeres and centromeres of chromosomes 3, 8, 9, 13, and 19 were studied by repeated fluorescence in situ hybridization, high-resolution cytometry, and three-dimensional image analysis in human blood lymphocytes before and after stimulation. Telomeres were found on the opposite side of the territories as compared with the centromeres for all chromosome territories investigated. Mutual distances between telomeres of submetacentric chromosomes were very short, usually shorter than centromere-to-telomere distances, which means that the chromosome territory is nonrandomly folded. Telomeres are, on average, much nearer to the center of the cell nucleus than centromeres; q-telomeres were found, on average, more centrally localized as compared with p-telotneres. Consequently, we directly showed that chromosome territories in the cell nucleus are (1) polar and (2) partially oriented in cell nuclei.
Permanent Link: http://hdl.handle.net/11104/0025271