Effects of adenosine on the growth of murine G:5:113 fibrosarcoma cells in vitro

0126993 - BFU-R 20033076 RIV CZ eng J - Journal Article
Hofer, Michal - Hoferová, Zuzana - Pospíšil, Milan - Znojil, V. - Chramostová, Kateřina
Effects of adenosine on the growth of murine G:5:113 fibrosarcoma cells in vitro.
Folia Biologica. Roč. 49, č. 5 (2003), s. 207-210. ISSN 0015-5500. E-ISSN 0015-5500
R&D Projects: GA ČR GA305/02/0423; GA AV ČR IBS5004009; GA AV ČR KSK5011112
Institutional research plan: CEZ:AV0Z5004920
Keywords : G:5:113 fibrosarcoma cell line * cell growth * adenosine
Subject RIV: BO - Biophysics
Impact factor: 0.527, year: 2003

It has been observed that adenosine suppresses the growth of G:5:113 murine fibrosarcoma cells in vitro with EC50 of 178 mM. Changes in the cell cycle including decreased percentage of cells in S-phase, increased portion of cells in G(0)/G(1)-phase, as well as prolonged generation time were found to be responsible for the growth suppression. Dipyridamole, a drug inhibiting the cellular uptake of adenosine, enhanced the growth suppression induced with adenosine in concentrations of 100 and 200 muM. It follows from these results that the action of adenosine on the G:5:113 cells is extracellular, mediated by adenosine receptors. Elevation of extracellular adenosine might serve potentially as an anticancer therapeutic agent.
Permanent Link: http://hdl.handle.net/11104/0025219