DNA interactions of monofuntional organometallic ruthenium(II) antitumor complexes in cell-free media

0126970 - BFU-R 20033053 RIV US eng J - Journal Article
Nováková, Olga - Chen, H. - Vrána, Oldřich - Rodger, A. - Sadler, P. J. - Brabec, Viktor
DNA interactions of monofuntional organometallic ruthenium(II) antitumor complexes in cell-free media.
Biochemistry. Roč. 42, č. 39 (2003), s. 11544-11554. ISSN 0006-2960
R&D Projects: GA ČR GA305/02/1552; GA ČR GA305/01/0418; GA AV ČR IAA5004101; GA MŠMT OC D20.002; GA MŠMT OC D20.005
Institutional research plan: CEZ:AV0Z5004920
Keywords : double-helical DNA * interstrand cross-links * biophysical analysis
Subject RIV: BO - Biophysics
Impact factor: 3.922, year: 2003

Modifications of natural DNA in a cell-free medium by antitumor monodentate Ru(II) arene compounds of the general formula [(6-arene)Ru(en)Cl]+ (arene = biphenyl, dihydroanthracene, tetrahydroanthracene, p-cymene, or benzene; en = ethylenediamine) were studied. The results indicate that these complexes bind preferentially to guanine residues in double-helical DNA. The data are consistent with DNA binding of the complexes containing biphenyl, dihydroanthracene, or tetrahydroanthracene ligands that involves combined coordination to G N7 and noncovalent interactions between the arene ligand and DNA. The single hydrocarbon rings in the p-cymene and benzene ruthenium complexes cannot interact with DNA by intercalation. It has been suggested that the different character of conformational alterations induced in DNA may affect differently further downstream effects of damaged DNA and may result in different biological effects of this new class of antitumor compounds.
Permanent Link: http://hdl.handle.net/11104/0025196