Recognition of DNA modified by antitumor cisplatin by "latent" and "active" protein p53

0126935 - BFU-R 20033016 RIV US eng J - Journal Article
Fojta, Miroslav - Pivoňková, Hana - Brázdová, Marie - Kovářová, Lucie - Paleček, Emil - Pospíšilová, Š. - Vojtěšek, B. - Kašpárková, Jana - Brabec, Viktor
Recognition of DNA modified by antitumor cisplatin by "latent" and "active" protein p53.
Biochemical Pharmacology. Roč. 65, č. 8 (2003), s. 1305-1316. ISSN 0006-2952. E-ISSN 1873-2968
R&D Projects: GA MZd NC5343; GA ČR GA204/02/0734; GA AV ČR IBS5004009; GA ČR GA305/02/1552; GA ČR GA305/01/0418; GA MZd NL6058; GA MZd NC7131; GA ČR GA301/02/0831; GA ČR GA301/00/P094
Institutional research plan: CEZ:AV0Z5004920
Keywords : tumor suppressor protein p53 * latent p53 * antitumor drugs
Subject RIV: BO - Biophysics
Impact factor: 2.993, year: 2003

Binding of active, latent and in vitro-activated p53 protein to DNA fragments globally modified by antitumor cisplatin was studied using electrophoretic mobility shift assay in agarose gels and immunoblotting analysis. Both latent and active p53 forms bound to platinated random sequences of DNA with a higher affinity than to unmodified DNA. The latent form exhibited a more pronounced selectivity for platinated DNA than the active p53.
Permanent Link: http://hdl.handle.net/11104/0025162