Electrochemical enzyme-linked immunoassay in a DNA hybridization sensor

0126749 - BFU-R 20023048 RIV NL eng J - Journal Article
Paleček, Emil - Kizek, René - Havran, Luděk - Billová, Sabina - Fojta, Miroslav
Electrochemical enzyme-linked immunoassay in a DNA hybridization sensor.
Analytica Chimica Acta. Roč. 469, č. 1 (2002), s. 73-83. ISSN 0003-2670. E-ISSN 1873-4324
R&D Projects: GA ČR GV204/97/K084; GA ČR GA204/00/D049
Institutional research plan: CEZ:AV0Z5004920
Keywords : electrochemical sensor for DNA hybridization * enzyme-linked immunoassay of target DNA * magnetic beads with attached DNA probe
Subject RIV: BO - Biophysics
Impact factor: 2.114, year: 2002

In most of the currently developed electrochemical DNA hybridization sensors short single-stranded probe DNA is immobilized on an electrode and both the hybridization and detection steps are carried out on the electrode surface. Here we use a new technology in which DNA hybridization is performed on commercially available magnetic beads and detection on solid electrodes. Paramagnetic Dynabeads Oligo(dT)25 (DBT) with covalently bound (dT)25 probe are used for the hybridization with target DNA containing adenine stretches.
Permanent Link: http://hdl.handle.net/11104/0024977