Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper

0126712 - BFU-R 20023010 RIV US eng J - Journal Article
Jelen, František - Josypčuk, Bohdan - Kouřilová, Alena - Novotný, Ladislav - Paleček, Emil
Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper.
Analytical Chemistry. Roč. 74, č. 18 (2002), s. 4788-4793. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA ČR GA203/02/0422; GA AV ČR IAA1163201; GA AV ČR IBS5004107
Institutional research plan: CEZ:AV0Z5004920
Keywords : hybridization * adenine * accumulation
Subject RIV: BO - Biophysics
Impact factor: 5.094, year: 2002

Electrochemical study is dealing with highly sensitive label-free techniques of DNA determination. DNA can be determined using copper solid amalgam electrodes or hanging mercury drop electrodes in the presence of copper. Small volumes of 5 - 3 muL of acid-treated DNA can easily be analyzed, thus making possible the determination of picogram and subpicogram amounts of DNA corresponding to attomole and subattomole quantities of 1000-base pair DNA. Application of this determination in DNA hybridization detection is demonstrated using surface H for the hybridization (superparamagnetic beads with covalently attached DNA probe) and the mercury electrodes only for the determination of DNA selectively captured at surface H.
Permanent Link: http://hdl.handle.net/11104/0024940