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Localization of Male-Specifically Expressed MROS Genes of Silene latifolia by PCR on Flow-Sorted Sex Chromosomes and Autosomes

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    0126464 - BFU-R 20013024 RIV US eng J - Journal Article
    Kejnovský, Eduard - Vrána, Jan - Matsunaga, S. - Souček, Přemysl - Široký, Jiří - Doležel, Jaroslav - Vyskot, Boris
    Localization of Male-Specifically Expressed MROS Genes of Silene latifolia by PCR on Flow-Sorted Sex Chromosomes and Autosomes.
    Genetics. Roč. 158, č. 3 (2001), s. 1269-1277. ISSN 0016-6731. E-ISSN 1943-2631
    R&D Projects: GA ČR GV521/96/K117; GA ČR GA521/99/0696; GA MŠMT ME 380
    Institutional research plan: CEZ:AV0Z5004920
    Keywords : Plant Melandrium-album * dioecious Silene * Y-chromosome
    Subject RIV: BO - Biophysics
    Impact factor: 4.803, year: 2001

    To localize the MROS (male-specifically expressed genes) in the dioecious Silene latifolia (white campion) flow-sorted chromosomes were used as a template for PCR with specific primers. Our results indicate that the MROS3 gene is located in at least two copies tandemly arranged on the X chromosome with additional copies on the autosomes, while MROS1, MROS2, and MROS4 are exclusively autosomal. Homology search of databases has shown the presence of five MROS3 homologues in Arabidopsis thaliana

    Permanent Link: http://hdl.handle.net/11104/0024693

     
     

Number of the records: 1  

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