Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding

0109714 - UMG-J 20043077 RIV US eng J - Journal Article
Čapková, Jana - Geussová, Gizela - Pěknicová, Jana
Acrosomal integrity of mammalian spermatozoa evaluated by monoclonal antibodies to intra-acrosomal proteins and role of these proteins in sperm-egg binding.
[Celistvost akrozomu savčích spermií hodnocená monoklonálními protilátkami proti vnitřním proteinům akrozomu a úloha těchto proteinů ve vazbě spermie a vajíčka.]
American Journal of Reproductive Immunology. Roč. 51, č. 6 (2004), s. 480-481. ISSN 1046-7408. E-ISSN 1600-0897.
[European congress of reproductive immunology. Plzeň, 30.06.2004-03.07.2004]
R&D Projects: GA ČR GA204/02/1373; GA MZd NJ7463
Keywords : monoclonal antibody * human spermatozoa * sperm protein
Subject RIV: EC - Immunology
Impact factor: 1.808, year: 2004

The integrity of sperm acrosome is required for normal sperm-egg interaction. Antibodies to acrosomal proteins are still frequently utilized to check the acrosomal state. We studied acrosomal proteins in boar and human spermatozoa by using of Hs-8 and Hs-14 monoclonal antibodies (MoAbs) which were prepared in our laboratory.The intra-acrosomal proteins recognized by Hs-8 and Hs-14 MoAbs are stable upon capacitation and even after induction of acrosome reaction. The role of pHs-8 and pHs-14 proteins in sperm-egg binding was investigated by in vitro binding assay of boar spermatozoa and porcine oocytes. The Hs-14 relevant protein does not participate in gamete binding while the Hs-8 detected protein is a secondary binding protein

Celistvost akrozomu spermie je nezbytná pro normální interakci spermie s vajíčkem. K ověření stavu akrozomu se ještě stále často používají protilátky proti proteinům akrozomu. Studovali jsme akrozomální proteiny pomocí protilátek Hs-8 a Hs-14 připravených v naší laboratoři. Proteiny uvnitř akrozomu rozpoznané Hs-8 a Hs-14 jsou při kapacitaci a dokonce i po indukci akrozomové reakce stabilní. Byla zkoumána úloha proteinů pHs-8 a pHs-14 ve vazbě spermie na vajíčko pomocí vazebného testu kančích spermií a prasečích oocytů in vitro. Protein zjištěny Hs-14 se vazby gamet neúčastní, zatímco protein detekovaný Hs-8 je proteinem sekundární vazby
Permanent Link: http://hdl.handle.net/11104/0016822