Microbiological aspects of determination of trichloroacetic acid in soil

0109150 - UEB-Q 20043073 RIV CZ eng J - Journal Article
Matucha, Miroslav - Gryndler, Milan - Uhlířová, H. - Fuksová, K. - Rohlenová, Jana - Forczek, Sándor - Schröder, P.
Microbiological aspects of determination of trichloroacetic acid in soil.
[Microbiological aspects of determination of trichloroacetic acid in soil.]
Folia Microbiologica. Roč. 49, č. 2 (2004), s. 117-122. ISSN 0015-5632. E-ISSN 1874-9356
R&D Projects: GA ČR GA522/02/0874
Institutional research plan: CEZ:AV0Z5038910
Keywords : NORWAY SPRUCE/SOIL-SYSTEM * HEADSPACE GAS-CHROMATOGRAPHY * BIODEGRADATION
Subject RIV: GK - Forestry
Impact factor: 1.034, year: 2004

Soils have been shown to possess a strong microbial trichloroacetic acid (TCA)-degrading activity. High TCA-degradation rate was also observed during soil extraction with water. For correct measurements of TCA levels in soil all TCA-degrading activities have to be inhibited immediately after sampling before analysis. We used rapid freezing of soil samples (optimally in liquid nitrogen) with subsequent storage and slow thawing before analysis as an efficient technique for suppressing the degradation. Frozen soil samples stored overnight at -20 degreesC and then thawed slowly exhibited very low residual TCA-degrading activity for several hours. Omitting the above procedure could lead to the confusing differences between the TCA levels previously reported in the literature.

Microbiological aspects of determination of trichloroacetic acid in soil
Permanent Link: http://hdl.handle.net/11104/0016260