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Affinity chromatography of bull seminal plasma proteins on mannan-Sepharose

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    0108978 - UMG-J 20043199 RIV IE eng J - Journal Article
    Liberda, J. - Ryšlavá, H. - Jelínková-Slavíčková, Petra - Jonáková, Věra - Tichá, M.
    Affinity chromatography of bull seminal plasma proteins on mannan-Sepharose.
    [Afinitní chromatografie proteinů býčí semenné plasmy na manan-Sepharose.]
    Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 780, č. 2 (2002), s. 231-239. ISSN 1570-0232. E-ISSN 1873-376X
    R&D Projects: GA ČR GA303/02/0433
    Institutional research plan: CEZ:AV0Z5052915
    Keywords : proteins * mannan
    Subject RIV: EB - Genetics ; Molecular Biology
    Impact factor: 1.913, year: 2002

    During fertilization sperm surface proteins interact with saccharide parts of the glycoprotein layer (ZP) of the ovum. To study the nature of the saccharide parts of ZP we used mannan containing mannose that was bound to Sepharose and after that the affinity was carried out and mannose-binding proteins were isolated. These proteins might probably participate in primary binding of the sperm with the ovum

    Během fertilizace povrchové proteiny spermií reagují se sacharidovými částmi glykoproteinové vrstvy (ZP) vajíčka. K prostudování vlastností sacharidových částí ZP jsme využili manan obsahující manózu vázaný na Sepharosu a poté jsme provedli afinitní chromatografii a izolovali jsme proteiny vázané na manan. Tyto proteiny by se mohly pravděpodobně účastnit primární vazby spermie na vajíčko
    Permanent Link: http://hdl.handle.net/11104/0016092

     
     

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