Number of the records: 1
Optimization of the host-plasmid interaction in the recombinant Escherichia coli strains overproducing penicillin G acylase
- 1.0108038 - MBU-M 20040244 RIV NL eng J - Journal Article
Valešová, Renata - Sobotková, Lenka - Štěpánek, Václav - Kyslík, Pavel
Optimization of the host-plasmid interaction in the recombinant Escherichia coli strains overproducing penicillin G acylase.
[Optimalizace vztahů "hostitel - plasmid" v rekombinantních kmenech Escherichia coli nadprodukujících penicilin-G acylázu.]
Enzyme and Microbial Technology. Roč. 35, - (2004), s. 74-80. ISSN 0141-0229. E-ISSN 1879-0909
Institutional research plan: CEZ:AV0Z5020903
Keywords : pga * overproduction * selection strategy
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.759, year: 2004
Segregational stability of the recombinant plasmid pKA18 bearing the pga gene encoding penicillin G acylase (PGA) and the production stability for PGA was studied in Escherichia coli strain W and its descendants. The hosts differ in the number of indigenous plasmids (pRK1–3) and the specific activity of PGA
U hostitelského kmene Escherichia coli W a z něho odvozených kmenů, lišících se počtem kryptických plazmidů (pRK1-3) a specifickou aktivitou penicilin-G-acylázy, byly studovány segregační stabilita rekombinantního plazmidu pKA18 nesoucího gen pga kódující penicilin-G-acylázu (PGA) a stabilita produkce PGA.
Permanent Link: http://hdl.handle.net/11104/0015178
Number of the records: 1